Question

The unknown will be added into the bathing fluid. Once this has been added to the reservoir and the tissue has been washed thoroughly, it is important to leave the tissue to equilibrate in the antagonist solution (for ~15 minutes) before repeating the agonist additions. This is:

A) to enhance the agonist effect

B) to inhibit the agonist effect

C) to neutralize the antagonist effect

D) to minimize the impact on the bathing fluid

Answer 1

Equilibrating tissue in an antagonist solution before reintroducing an agonist allows the tissue to adjust to the antagonist, serving to inhibit the agonist effect when the agonist is added again.

Step-by-step explanation:

The question refers to the procedure where an antagonist solution is introduced to a tissue sample after exposure to an agonist. The period of equilibration in the antagonist solution serves to allow the tissue to adjust to the presence of the antagonist so that it can effectively inhibit the agonist effect when the agonist is reintroduced. This step does not enhance the agonist effect, neutralize the antagonist effect, or is meant to minimize the impact on the bathing fluid directly; rather, it is a necessary phase to ensure the biological tissue has reached a new equilibrium with the antagonist before further experimentation. It's important in pharmacology and physiology experiments to accurately gauge the impact of various substances on biological tissues.