Final answer:
During translation, the ribosome moves along the mRNA in three steps facilitated by elongation factor-G (EF-G). In the first step, the ribosome moves one codon with the help of EF-G and GTP. Then, the dipeptide is shifted from the A site to the P site while the deacylated tRNA is released. This process results in the movement of the codons and tRNA along the mRNA.
Step-by-step explanation:
During translation, there are three main steps involved in the movement of the ribosome along the mRNA. These steps are facilitated by the action of various factors, including elongation factor-G (EF-G), also known as translocase.
- First, the ribosome moves along the mRNA by a distance of one codon (three bases) with the help of EF-G and GTP. This step is known as translocation.
- During translocation, the dipeptide (two amino acids) is shifted from the A site to the P site, along with its tRNA. At the same time, the deacylated tRNA (the first tRNA) is released into the cytosol.
- As a result of translocation, the third codon of the mRNA is now at the A site, and the second codon attached to tRNA with the peptide is in the P site.