Final answer:
To locate small objects on a slide, start with the lower magnification (low power) to find objects and then switch to higher magnification (high power) for detailed observation. Use the coarse focusing knob for larger adjustments on low power and the fine focusing knob for detailed focusing on high power.
Step-by-step explanation:
To locate small objects on a slide first find them under low power; field before rotating to high power.
When using a microscope, it is crucial to start with a lower magnification to get a broader view of the specimen. This allows for easier location of the objects of interest on the slide.
Once the objects are centered under the low power objective (typically 4x or 10x), the user can then switch to a higher magnification objective (such as 40x or 100x) to get a more detailed look at the specimen. This technique also decreases the risk of the slide or objective lens getting damaged, as the higher magnification lenses come closer to the slide and require more precise focusing.
Additionally, when switching to a higher power lens, the coarse focusing knob should not be used due to the sensitivity and potential damage it could cause because of its large-scale adjustments. Instead, the fine focusing knob should be used for precise focusing, making minute adjustments to the focus while using the high power objective.