Final answer:
Energy is a major factor that limits the number of trophic levels in a food chain.
Step-by-step explanation:
One major factor that limits the number of steps in a food chain is energy. Energy is lost at each trophic level and between trophic levels as heat and in the transfer to decomposers. Thus, after a limited number of trophic energy transfers, the amount of energy remaining in the food chain may not be great enough to support viable populations at higher trophic levels.