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What are the ingredient in these agars plates that inhibit the growth of gram gram +?

1.Nutrient Agar
2.MacConkey Agar (2)
3.EMB Agar (2)
4.XLD Agar

User Priyadi
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1 Answer

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Final answer:

The selective agents present in the agar inhibit the growth of Gram-positive bacteria in these agar plates.

Step-by-step explanation:

The ingredient in these agar plates that inhibit the growth of Gram-positive bacteria are the selective agents present in the agar.

1. Nutrient Agar does not contain specific ingredients that inhibit the growth of Gram-positive bacteria.

2. MacConkey Agar contains bile salts and crystal violet which inhibit the growth of most Gram-positive, non-enteric bacteria.

3. EMB Agar contains eosin and methylene blue dyes that inhibit the growth of Gram-positive bacteria.

4. XLD Agar contains selective agents like bile salts and phenol red that inhibit the growth of Gram-positive bacteria.

Ingredients such as bile salts and crystal violet in media like MacConkey Agar inhibit the growth of gram-positive bacteria, favoring gram-negative bacteria. These components of selective media create an environment that is hostile to gram-positive bacteria due to the disruption of their cell wall structure. On the other hand, EMB Agar, which contains indicator dyes eosin and methylene blue, also suppresses gram-positive bacteria and differentiates between lactose fermenters and non-lactose fermenters by the color of the colonies formed. EMB agar is an example of a selective medium in microbiology.

User Lptr
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