Final answer:
Proteins in serum can be separated using methods like differential centrifugation, size exclusion chromatography, and electrophoresis techniques such as SDS-PAGE and immunofixation.
Step-by-step explanation:
To separate different proteins in serum, various techniques can be employed. The process often involves the use of differential centrifugation, size exclusion chromatography, and electrophoresis, including SDS-PAGE and immunoelectrophoresis. Among these methods, SDS-PAGE separates proteins based on their molecular weight after binding to SDS, which imparts a negative charge proportionate to their size. Another technique, immunofixation, identifies specific proteins using antibodies. In practice, serum proteins can also be precipitated with salts or using a semi-permeable membrane under centrifugation to separate bound and unbound fractions.
We separate proteins in serum using differential centrifugation, size exclusion chromatography, and various forms of electrophoresis techniques.