Final answer:
DNA cloning predominantly requires the use of DNA ligase to join DNA fragments and restriction enzymes to cut DNA at specific sequences. DNA polymerase is not uniformly required in all cloning methods.
Step-by-step explanation:
DNA cloning involves several steps where different enzymes play crucial roles. The first step usually involves using restriction enzymes to cut DNA at specific sequences, which facilitates the insertion of the DNA fragment of interest into a vector. Next, DNA ligase is critical as it seals the DNA fragments by forming phosphodiester bonds, thus joining the inserted DNA with the vector DNA to complete the recombinant DNA molecule. Although DNA polymerase is crucial for DNA replication and can be used in some cloning techniques (e.g., PCR cloning), it is not a compulsory enzyme uniformly required across all DNA cloning methods as the initial question suggests.
Therefore, the correct answer to the question 'DNA Cloning requires the use of which of the following enzymes? I. DNA Polymerase II. DNA Ligase III. Restriction Enzymes' is (D) II and III Only since both DNA ligase and restriction enzymes are critical in the process of DNA cloning.