Question

DNA and RNA range for the UV range is around what?

1) 200-300 nm
2) 300-400 nm
3) 400-500 nm
4) 500-600 nm

Answer 1

DNA and RNA range for the UV range is around 200-300 nm (option 1).

Step-by-step explanation:

DNA and RNA exhibit maximal absorbance in the UV range of approximately 200-300 nanometers (option 1). This specific wavelength range is often referred to as the UV-C range. The absorption of UV light by nucleic acids is primarily attributed to the presence of aromatic nucleotide bases, such as adenine, thymine, cytosine, and guanine.

The absorption of UV light by DNA and RNA is a fundamental principle used in laboratory techniques such as nucleic acid quantification and analysis. The absorption spectra of DNA and RNA show distinct peaks in the UV-C range, allowing researchers to accurately measure the concentration of nucleic acids in a sample. The absorption of UV light induces electronic transitions in the nucleotide bases, providing a sensitive and reliable method for the detection and characterization of nucleic acids.

Understanding the UV absorbance properties of DNA and RNA is essential in various scientific disciplines, including molecular biology, genetics, and biochemistry, where precise quantification and analysis of nucleic acids are integral to experimental procedures.