Question

Generating the Standards for the Curve, 5 test tubes were labeled numerically. Using a 200uL pipette the calculated concentrations of BSA stock and water were added to each tube. What is the purpose of labeling the test tubes numerically?

1) To keep track of the different concentrations of BSA stock and water in each tube
2) To ensure that the correct amount of BSA stock and water is added to each tube
3) To identify the tubes during the experiment
4) To create a standard curve for the experiment

Answer 1

Numerical labeling of test tubes is essential for tracking different concentrations of solutions, ensuring correct volumes are added, and identifying tubes during an experiment to create a standard curve.

Step-by-step explanation:

The purpose of labeling the test tubes numerically is threefold. First, it helps in tracking the different concentrations of BSA (bovine serum albumin) stock and water added to each tube, which is crucial for reproducibility and accuracy in the experiment. Second, it aids in ensuring that the correct volumes of BSA stock and water are added, as each tube might require specific amounts based on the desired final concentration. Third, it serves as an identifier during the experiment, letting the experimenter easily match each tube to its corresponding data point in the standard curve or other results.

These labeled test tubes are used to create a standard curve, which is a graph displaying the relationship between the concentration of the BSA and a measurable property (like absorbance in spectroscopy). The standard curve is then used to determine the concentration of BSA in unknown samples by comparing their properties to the curve.