Final answer:
To evaluate the purity of mutant ykkCD sensor RNA, compare gel electrophoresis bands before and after purification, where fewer bands post-purification indicate higher purity.
Step-by-step explanation:
To evaluate the purity of your mutant ykkCD sensor RNA, one would compare the bands visible on a gel electrophoresis before and after purification. Bands represent RNA fragments and their intensities can be indicative of concentration and purity. The number of bands in the Pure vs Lysate will reflect this.
In the lysate, you might expect multiple bands indicating various RNA species and impurities. After purification, you would hope to see fewer or a single band representing the purified ykkCD sensor RNA. A decrease in the number of bands post-purification suggests that other RNA species and impurities have been removed, thus indicating increased purity. The sharpness and distinctness of the bands also play a role in assessing purity – with sharper, more discrete bands generally signifying higher purity levels of the RNA of interest.