Final answer:
GST tag is a protein tag used in molecular biology techniques to purify proteins. It binds to a resin column, and the protein is eluted from the column. To remove the GST tag, enzymatic cleavage using a specific protease can be performed.
Step-by-step explanation:
GST tag is a protein tag that is commonly used to purify proteins in molecular biology techniques. It binds to a specific resin, allowing for the purification of the protein of interest. To purify a GST-tagged protein, the lysate containing the protein is passed through a resin column that specifically binds to the GST tag. The protein is then eluted from the column using a high concentration of the elution buffer.
To remove the GST tag from the purified protein, an enzymatic cleavage method can be employed. The specific protease recognizes a recognition sequence within the GST tag and cleaves it, leaving the protein of interest free from the tag. The cleavage reaction can be performed under specific conditions, such as temperature and pH, to ensure optimal enzyme activity.