Final answer:
To determine the significance of the hypothesis that pre-natal exposure to hormones increases the number of females in midshipman fish populations, a sign test can be used to calculate the probability of observing 4 or more females out of 6 births. If this probability is less than the significance level, the hypothesis is supported.
Step-by-step explanation:
To test the hypothesis that exposure to hormones will increase the number of females in the midshipman fish population, a sign test can be used. In this case, the researcher exposed a group of pregnant midshipman fish to birth control hormones and recorded the sex of the first 6 offspring. Out of the 6 offspring, 4 were female. To calculate the significance using a sign test, we determine the probability of observing 4 or more females out of 6 births if the population sex makeup is 50% female. If this probability is less than a predetermined significance level (usually 0.05), we reject the null hypothesis and conclude that the exposure to hormones increases the number of females in the population.