Question

The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating the sample 15 min at room temp.

Calculate the protein concentration in the sample for a tube using 0.3 mL of the 2.0 mg/mL standard BSA.

Answer 1

The Biuret protein assay is a method used to measure the concentration of proteins in a sample. The protein concentration in the sample can be calculated using a standard BSA solution and the absorbance readings.

Step-by-step explanation:

The Biuret protein assay is a method used to measure the concentration of proteins in a sample. In this assay, a protein sample is mixed with Biuret reagent and the resulting mixture is incubated. The amount of protein present is then determined by measuring the absorbance at a specific wavelength.

To calculate the protein concentration in the sample using a standard BSA solution, you can use the following formula:

(Absorbance of sample / Absorbance of standard) x Concentration of standard