Final answer:
To prepare 300 mL of 1X TAE from a 10X stock solution, you would need to mix 30 mL of the 10X stock with 270 mL of water.
Step-by-step explanation:
To make 300 mL of 1X TAE from a 10X TAE stock, you would need to dilute it 1:10. This means that for every part of the 10X stock, you need to add 9 parts of water. Therefore, to find the amount of 10X stock required for 300 mL of 1X, you would divide 300 mL by 10 to find how much of the 10X stock is needed and then subtract this volume from the total 300 mL to calculate the volume of water needed.
Here's the calculation:
- Volume of 10X TAE stock needed = 300 mL / 10 = 30 mL
- Volume of water needed = 300 mL - 30 mL = 270 mL
Therefore, you would need 270 mL of water to make 300 mL of 1X TAE from the 10X TAE stock.