Question

Determine the mass of tris to be measured to make 100 ml of 10mm tris solution? describe the steps. this solution has be have a ph of 7.5

Answer 1

To prepare a 10 mM Tris solution with pH 7.5, dissolve 1.2114 g of Tris in less than 100 mL of Millipore water, adjust the pH as needed, and dilute to the final volume of 100 mL.

Step-by-step explanation:

To determine the mass of Tris (tris(hydroxymethyl)aminomethane) needed to prepare 100 mL of a 10 mM solution with a pH of 7.5, you would first calculate the molar mass of Tris, which is approximately 121.14 g/mol. Then, use the formula: Mass of Tris (g) = Molarity (M) × Volume (L) × Molar Mass (g/mol)

For a 10 mM (0.010 M) solution: Mass of Tris (g) = 0.010 mol/L × 0.100 L × 121.14 g/mol = 1.2114 g

Since the desired pH is 7.5, adjustments using HCl and NaOH may be necessary to reach the desired pH after dissolving Tris in Millipore water. Transfer the solution to a volumetric flask, adjust the pH if needed, and dilute to the final volume with Millipore water.