Question

Choose the steps in the synthesis of the tetrapeptide ala−ile−met−gly, using Merrifield's method.

a) Deprotection, coupling, activation, resin cleavage
b) Activation, deprotection, resin cleavage, coupling
c) Cleavage, activation, coupling, deprotection
d) Cleavage, deprotection, activation, coupling

Answer 1

The correct sequence of steps in Merrifield's method for synthesizing the tetrapeptide ala−ile−met−gly is Deprotection, Coupling, Activation, and Resin Cleavage.

Step-by-step explanation:

The steps in the synthesis of the tetrapeptide ala−ile−met−gly using Merrifield's method involve multiple stages including the deprotection of the amino acid, the coupling of amino acids, and activation for the next amino acid addition, with the final stage being the cleavage from the resin.

1. Deprotection - Removal of protective groups from the amino acids.
2. Coupling - Formation of peptide bonds between amino acids.
3. Activation - Preparing the next amino acid for coupling.
4. Resin Cleavage - Releasing the completed peptide from the solid support.

Therefore, the correct sequence for the synthesis is Deprotection, Coupling, Activation, Resin Cleavage, which corresponds to option (a).