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Briefly explain how site-directed mutagenesis is carried out.

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Final answer:

Site-directed mutagenesis is a technique used in molecular biology to make specific changes to a DNA sequence. The QuickChange process allows scientists to specify exactly where mutations will occur in the DNA.

Step-by-step explanation:

Site-directed mutagenesis is a technique used in molecular biology to make specific changes to a DNA sequence. It allows scientists to specify exactly where mutations will occur in the DNA. The QuickChange process is one method of performing site-directed mutagenesis, and it involves designing specific primers that target the desired mutation sites and using PCR to amplify and replicate the DNA with the desired mutations. The QuickChange Site-Directed Mutagenesis method simplifies this process, allowing for the alteration of a few nucleotides within a DNA sequence. Unlike conventional methods which may require digestion with restriction endonucleases or ligation, QuickChange does not, thus reducing the time needed for mutagenesis significantly.

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