Question

A 250-μl sample is needed from a sterile vessel that is too thin to use with anything but a sterile pipet. Which pipet and pipet pump could be used to withdraw the mL equivalent of 250 μL from the vessel?

Answer 1

Use a 20-200 μl micropipette along with a sterile pipette pump to accurately withdraw 250 μl from a thin sterile vessel, while practicing aseptic techniques.

Step-by-step explanation:

To withdraw the milliliters equivalent of 250 μl from a sterile vessel using sterile techniques, you should use a micropipette capable of dispensing volumes in that range. Specifically, a micropipette that can adjust between 20-200 μl would be suitable for accurately dispensing 250 μl, as this is within its volume range. The corresponding pipet pump, often referred to as a pipettor or pipette aid, must be sterile as well and is used to draw the desired amount of liquid into the micropipette. It is recommended to practice aseptic techniques, such as flaming the vessel's opening and keeping it angled, to minimize the risk of contamination.