Final answer:
The protein that binds the two separated DNA strands to prevent them from base pairing again before replication is called single-stranded binding protein (SSB).
Step-by-step explanation:
The protein that binds the two separated DNA strands to prevent them from base pairing again before replication is called single-stranded binding protein (SSB).
SSBs coat the DNA strands to keep them separated and prevent them from reannealing.