Question

How might one determine the influence of CAAT boxes on the transcription rate of a given gene?

a. Delete the CAAT box sequence and measure the transcription rate.
b. Make mutations in the CAAT box sequence and measure the transcription rate.
c. Introduce extra CAAT box sequences and measure the transcription rate.
d. All of the above would be good ways to determine the influence of CAAT boxes on transcription rate.

Answer 1

To assess the CAAT boxes' effect on transcription rate, one could delete, mutate, or introduce extra CAAT box sequences and then measure the transcription rate.

Step-by-step explanation:

To determine the influence of CAAT boxes on the transcription rate of a given gene, one might:

1. Delete the CAAT box sequence and measure the transcription rate.
2. Make mutations in the CAAT box sequence and measure the transcription rate.
3. Introduce extra CAAT box sequences and measure the transcription rate.

Each of these methods corresponds to how mutated promoters and the binding sites for transcription factors can affect the gene's transcription. By altering the CAAT box which is an essential eukaryotic promoter sequence involved in binding transcription factors, researchers can observe changes in transcription levels (whether an increase, decrease, or no change at all).