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PhiX174 replication: Which of the following is NOT a function of Protein A?

A. Site-specific DNase (nicking of one strand)
B. Recognition of the origin
C. Single-strand ligase
D. Covalently link to the 5-end of single-strand DNA
E. Nuclear localization

User Hyo
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1 Answer

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Final answer:

Protein A of PhiX174 is involved in the recognition of the origin of replication, nicking DNA, and helicase activity but not in ligase function or nuclear localization.

Step-by-step explanation:

Protein A of PhiX174 plays a vital role in the replication process of the virus, but not all listed functions are associated with Protein A. Specifically, Protein A is responsible for the site-specific DNase activity, which involves the nicking of one strand of DNA. It also recognizes the origin of replication, is covalently linked to the 5'-end of single-strand DNA, and acts as a helicase by separating DNA strands. However, one function that is not associated with Protein A is acting as a single-strand ligase, and it does not have a nuclear localization since PhiX174 is a bacteriophage and infects bacteria, which lack a defined nucleus.

User Toki
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