Question

After completion of the electrophoresis of DNA fragments along with proper molecular weight standards on an agarose gel, suppose the gel is blank (no bands, no molecular weight standard). What might be the explanations?

1) The DNA fragments did not migrate properly
2) The gel was not prepared correctly
3) The electrophoresis buffer was not prepared correctly
4) The power supply was not functioning properly

Answer 1

Possible explanations for a blank agarose gel with no bands or molecular weight standards after electrophoresis, the gel is blank with no bands or molecular weight standards, several explanations could be possible:

Step-by-step explanation:

If after completion of the electrophoresis of DNA fragments along with proper molecular weight standards on an agarose gel, the gel is blank with no bands or molecular weight standards, several explanations could be possible:

1. The DNA fragments did not migrate properly.
2. The gel was not prepared correctly.
3. The electrophoresis buffer was not prepared correctly.
4. The power supply was not functioning properly.