Question

Which of the following accurately depicts where the fluorescence should be localized in the cells when looking at the treated cultures using fluorescence microscopy?

a) Endoplasmic reticulum
b) Golgi apparatus
c) Microtubules
d) Actin filaments

Answer 1

Fluorescence microscopy allows for the specific visualization of cellular structures by staining them with fluorescent dyes; thus, microtubules would be green, actin microfilaments red, keratin yellow, and the Golgi apparatus orange.

Step-by-step explanation:

According to the information provided, fluorescence microscopy is used to visualize different structures within eukaryotic cells by staining these structures with fluorescent dyes. The fluorescence localization depends on the target of the specific fluorophore-tagged antibodies used to stain the cell components. In this case:

• Microtubules are depicted as green
• Actin microfilaments show up as red
• The nucleus appears blue
• Keratin, a type of intermediate filament, is yellow
• Golgi apparatus is stained with an orange fluorescent dye

Therefore, if the treated cultures under examination have used the appropriate fluorescent stains, the fluorescence should be localized to the structures that correspond with the color of the stain mentioned: microtubules in green, actin filaments in red, and the Golgi apparatus in orange.