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Uracil- containing nucleotides may be utilized in the reaction catalyzed by which of the following enzymes?

A. DNA polymerase
B. Primase and RNA polymerase
C. DNA ligase
D. DNA helicase
E. DNA primase
F. DNA topoisomerase

1 Answer

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Final answer:

Primase and RNA polymerase are the enzymes that utilize uracil-containing nucleotides. Primase is involved in RNA primer synthesis for DNA replication, while RNA polymerase is responsible for RNA transcription, which includes uracil bases.

Step-by-step explanation:

The enzymes capable of utilizing uracil-containing nucleotides are primase and RNA polymerase. Primase synthesizes RNA primers during DNA replication which are necessary starting points for DNA Polymerases to begin synthesis of the new DNA strand. RNA polymerase, on the other hand, is responsible for transcribing RNA from a DNA template, which includes messenger RNA (mRNA), ribosomal RNA (rRNA), and transfer RNA (tRNA), all of which contain uracil rather than thymine.

DNA polymerase indeed replaces the RNA primer nucleotides with DNA nucleotides during the DNA replication process. However, the initial RNA primer is synthesized by primase, which incorporates uracil into its RNA structure. Furthermore, DNA polymerase I plays a role in removing the RNA primer and replacing its nucleotides with DNA.

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