Final answer:
To extract a transmembrane tyrosine kinase and assay its function, you would use a detergent that can solubilize and stabilize the membrane protein. Nonionic detergents, such as octylglucoside, and ionic detergents, such as sodium deoxycholate, are commonly used for this purpose.
Step-by-step explanation:
To extract a transmembrane tyrosine kinase and assay its function, you would use a detergent that can solubilize and stabilize the membrane protein. Nonionic detergents, such as octylglucoside, and ionic detergents, such as sodium deoxycholate, are commonly used for this purpose.
Nonionic detergents have uncharged hydrophilic headgroups and are based on polyoxyethylene or a glycoside. Examples include Tween, Triton, and the Brij series. Ionic detergents, on the other hand, have charged headgroups and can disrupt protein-protein interactions. Examples include sodium deoxycholate and other sulfates and sulfonates.
Tide laundry detergent is not suitable for extracting a transmembrane tyrosine kinase as it is designed for cleaning clothes and contains a mixture of surfactants that are not specifically optimized for protein extraction.