Question

You need to examine a fluorophore on the microscope. It requires Orange excitation light. What wavelength do you expect the fluorophore to emit?

a. Green light
b. Yellow light
c. Red light
d. Blue light
e. Microwaves

Answer 1

When a fluorophore absorbs orange excitation light, it emits light at a longer wavelength, which will be in the red spectrum of the visible light. Hence, the correct emission color is red light.

Step-by-step explanation:

The question you've asked is about fluorescence microscopy and the emission wavelength of a fluorophore that requires orange excitation light. Fluorescent compounds absorb light at one wavelength (the excitation wavelength) and then emit light at a longer wavelength, which is the emission wavelength. In fluorescence microscopy, when a fluorophore is excited by orange light, we can expect it to emit light in a color with a longer wavelength, which means it will emit red light.

Fluorescence is a natural phenomenon that is used in microscopy to create contrast between structures or molecules within a specimen. The excitation light, generally a form of electromagnetic radiation with a short wavelength like ultraviolet or blue light, is absorbed by the fluorophores and re-emitted as visible light with a longer wavelength.

In summary, if a fluorophore absorbs orange light, which is in the middle of the visible spectrum, its emission is going to be at a longer wavelength, hence in red light, which is the correct answer: c. Red light.