Question

What volume of water is needed to make 40 uL of 100 ug/mL BSA from a 2mg/mL BSA stock?

Answer 1

To make 40 uL of 100 ug/mL BSA from a 2mg/mL BSA stock, add 2 mL of water to 40 uL of the stock.

Step-by-step explanation:

To calculate the volume of water needed to make 40 uL of a 100 ug/mL BSA solution from a 2mg/mL BSA stock, we can use the formula:

V1C1 = V2C2

Where V1 is the volume of the stock solution, C1 is the concentration of the stock solution, V2 is the volume of the final solution, and C2 is the concentration of the final solution.

Using this formula, we can rearrange to solve for V1:

V1 = (V2C2) / C1

Plugging in the values, we get:

V1 = (40 uL * 100 ug/mL) / (2 mg/ml) = 2000 uL = 2 mL

So, you would need to add 2 mL of water to 40 uL of the 2mg/mL BSA stock to make 40 uL of 100 ug/mL BSA solution.