Question

Put the following steps in order to indicate a correct broth to broth tube transfer:

1) Sterilize the broth tube and broth transfer loop
2) Flame the neck of the broth tube
3) Insert the loop into the broth tube and transfer a small amount of broth
4) Flame the neck of the broth tube again
5) Close the broth tube tightly

Answer 1

The correct order for a broth to broth tube transfer is to sterilize the broth tube and broth transfer loop, flame the neck of the broth tube, insert the loop into the broth tube and transfer a small amount of broth, flame the neck of the broth tube again, and close the broth tube tightly.

Step-by-step explanation:

1. Sterilize the broth tube and broth transfer loop: This step is important to ensure that no contaminants are introduced into the broth during the transfer process.
2. Flame the neck of the broth tube: By briefly passing the opening of the tube through a flame, airborne contaminants can be discouraged from entering the sterile liquid.
3. Insert the loop into the broth tube and transfer a small amount of broth: Using a flame-sterilized inoculating loop, pick up a small amount of the broth and transfer it to the tube.
4. Flame the neck of the broth tube again: After the transfer, flaming the neck of the broth tube once more helps to further prevent contamination.
5. Close the broth tube tightly: Finally, close the broth tube tightly to maintain sterility.