Question

The numbered events listed below participate in the gen- eration of junctional diversity. Put them in chronological order.

a. DNA strands pair, and unpaired nucleotides are removed by exonuclease activity.
b. P-nucleotides are generated after nicking of one DNA strand.
c. DNA polymerase fills in gaps, and DNA ligation forms a coding joint.
d. The RAG complex cleaves heptamer RSSs, and DNA hairpins are formed.
e. Terminal deoxynucleotidyl transferase adds N-nucleotides to the 3ʹ end of the stretch of P-nucleotides.

Answer 1

The events that participate in the generation of junctional diversity are the RAG complex cleaving heptamer RSSs, terminal deoxynucleotidyl transferase adding N-nucleotides, P-nucleotides being generated after nicking of one DNA strand, DNA strands pairing and unpaired nucleotides being removed, and DNA polymerase filling in gaps and DNA ligation forming a coding joint.

Step-by-step explanation:

The events that participate in the generation of junctional diversity in chronological order are as follows:

1. d. The RAG complex cleaves heptamer RSSs, and DNA hairpins are formed.
2. e. Terminal deoxynucleotidyl transferase adds N-nucleotides to the 3ʹ end of the stretch of P-nucleotides.
3. b. P-nucleotides are generated after nicking of one DNA strand.
4. a. DNA strands pair, and unpaired nucleotides are removed by exonuclease activity.
5. c. DNA polymerase fills in gaps, and DNA ligation forms a coding joint.