Question

Why would phe have a higher rf in ion exchange chromatography?

Option 1: Phenylalanine (Phe) has a stronger affinity for the stationary phase, resulting in higher retention.

Option 2: Phe interacts more weakly with the mobile phase, leading to increased retention.

Option 3: Ion exchange favors aromatic compounds like Phe, causing higher retention.

Option 4: Phe experiences increased ion exchange interactions, causing slower elution.

Answer 1

In ion exchange chromatography, Phenylalanine (Phe) would have a higher retention factor due to its stronger affinity for the stationary phase.

Step-by-step explanation:

In ion exchange chromatography, the affinity of a compound for the stationary phase determines its retention on the column. In this case, Phenylalanine (Phe) would have a higher retention factor (rf) in ion exchange chromatography because it has a stronger affinity for the stationary phase (option 1). The compounds that have a stronger affinity for the stationary phase will be retained longer, resulting in higher rf values.