Question

Calculate the amount of agarose and 10,000x Sybr Safe needed to make 15 ml of 1% agarose 1x Sybr Safe.

Option 1: 0.15 g agarose, 0.015 ml Sybr Safe
Option 2: 1.5 g agarose, 0.15 ml Sybr Safe
Option 3: 0.75 g agarose, 0.075 ml Sybr Safe
Option 4: 2.0 g agarose, 0.2 ml Sybr Safe

Answer 1

To make 15 ml of 1% agarose 1x Sybr Safe, you would need 0.15 g of agarose and 0.015 ml of 10,000x Sybr Safe.

Step-by-step explanation:

To calculate the amount of agarose and 10,000x Sybr Safe needed to make 15 ml of 1% agarose 1x Sybr Safe, you need to determine the correct amounts for both components. A 1% agarose gel means that for every 100 ml of solution, there is 1g of agarose. Therefore, for 15 ml of solution, you would need 0.15 g of agarose. As for the Sybr Safe, a 10,000x concentration means that you would need to dilute it 10,000 times. For a final volume of 15 ml, you would need 0.015 ml of the 10,000x Sybr Safe.