Final answer:
Yes, serial dilution is used to create solutions of known concentration that can be measured by a spectrophotometer.
Step-by-step explanation:
Yes, serial dilution is used to create solutions of known concentration that can be measured by a spectrophotometer.
Serial dilution involves diluting a fixed volume of a stock solution with a dilution solution, creating a series of diluted solutions with decreasing concentrations. Each dilution in the series is typically a fixed factor lower than the previous dilution. By performing serial dilution, a range of known concentrations can be obtained.
The diluted solutions can then be measured using a spectrophotometer, which measures the absorbance or transmittance of the solution at a specific wavelength. The spectrophotometer provides data that can be used to determine the concentration of the original stock solution.