Final answer:
Most of the potential energy from glucose after glycolysis is retained in the resulting two pyruvate molecules, while a portion is used to produce ATP and NADH through substrate-level phosphorylation.
Step-by-step explanation:
At the end of glycolysis, most of the potential energy from glucose is retained in the two pyruvates. During the process of glycolysis, a glucose molecule is broken down into two pyruvate molecules. Each pyruvate retains a significant portion of the energy that was originally in the glucose molecule, while a small amount of energy is used to produce ATP directly by substrate-level phosphorylation and to reduce NAD+ to form NADH. In this process, a net gain of two ATP molecules is produced, but more energy is still contained within the two pyruvate molecules that are formed.