To create a Lineweaver-Burk plot in Excel, enter reciprocal substrate concentration and velocity data, insert a scatter plot, add a linear trendline, and use the trendline's equation to determine Km and Vmax values.
Input your reciprocal substrate concentration (1/[S]) in one column and the reciprocal velocity (1/v) in another column.
Select the data and insert a scatter plot.
Use the 'Add Trendline' function to add a linear trendline to the scatter plot.
Format the trendline to display the equation on the chart. This equation will provide the values of Km and Vmax based on the slopes and intercepts.
Note: The Lineweaver-Burk plot is a double reciprocal graph that can be used to determine important enzymatic parameters like Km and Vmax.