Final answer:
The minor spliceosome recognizes AU at the 5' splice site and branch site during splicing of pre-mRNA molecules.
Step-by-step explanation:
During RNA splicing, the minor spliceosome is in charge of identifying non-canonical splice sites. The minor spliceosome recognises AU at the 5' splice site as opposed to GU, as does the major spliceosome.
One distinctive aspect of the minor spliceosome's activity is its detection of the AU-AC splice site. Rare, unique introns with non-canonical splice sites are removed by the minor spliceosome.
The recognition of AU at the 5' splice site by the minor spliceosome emphasises its function in processing particular introns with distinct sequence features during pre-mRNA splicing.