Question

Whys is it important for the Overdrive element to be located next to the Right Border instead of the Left Border? (Read the answers carefully.) -This ensures that the right border is cut first. Otherwise the entire T-DNA region would stay in the bacteria instead of being transferred. -Overdrive ensures that the VirD1 nicks at the correct site. -This location ensures that the T-strand gets shipped to the plant nucleus. -This location facilitates the recruitment of VirE2 to the newly replicated T-strand.

Answer 1

The important for the Overdrive element to be located next to the Right Border instead of the Left Border is C) This location ensures that the T-strand gets shipped to the plant nucleus.

Step-by-step explanation:

The T-strand is a single strand of DNA that needs to be transported from the bacterium to the plant nucleus in order for successful gene transfer to occur. Plasmids, such as the Ti plasmid in Agrobacterium, contain the T-DNA region which carries the genes to be transferred. The Overdrive element is a specific sequence within the T-DNA region that is crucial for transport of the T-strand to the plant nucleus.

The Overdrive element acts as a signal for the transport machinery in the plant cells, directing it to transport the T-strand to the nucleus. This element is recognized by a protein called VirE2, which is part of the transport machinery. The Overdrive element being located next to the Right Border ensures that the VirE2 protein is recruited to the newly replicated T-strand effectively.

In conclusion, the location of the Overdrive element next to the Right Border is important because it facilitates the recruitment of VirE2 to the newly replicated T-strand, ensuring that the T-strand gets shipped to the plant nucleus. The correct answer is C) This location ensures that the T-strand gets shipped to the plant nucleus.