Answer: You have not given the dilution ratio. Based on the information you have given here the answer is .25 mg/mL.
Explanation: The dilution factor or the dilution is the initial volume divided by the final volume.
DF=Vi/Vf - but three times (three test tubes)
Your dilution factor is 1/4 X 1/4 X 1/4 = 1/64
1/64 X 16mg/mL = 16/64 mg/mL or 1/4 mg/mL