Question

Which of the following statements are INCORRECT:

OA. Genomic DNA contains introns and exons.
B. During the process of protein production an RNA copy is made from small sections of the genomic DNA.
OC. Messanger RNA (mRNA) contains only the coding regions, introns are spliced out.
D. Translation is the synthesis of protein, a process which occurs in the nucleus of the cell.
OE. Codons in mRNA are read during translation, beginning at a start codon and continuing until a stop codon
is reached.

QUESTION 5
Which of the following statement is INCORRECT?
A. PCR can be used to produce many copies of a specific gene.
B. A PCR cycle includes denaturation, annealing and extension steps.
C. DNA is completely denatured at 72°C.
D. PCR is a doubling reaction and starting with a single strand of DNA, after the 10th cycle of a PCR
reaction you will have 1024 copies.
OE. PCR requires a forward and a reverse primer.

Answer 1

For Question 4:
Option D is INCORRECT. Translation is the process of protein synthesis that occurs in the cytoplasm of the cell, not in the nucleus.

For Question 5:
Option C is INCORRECT. DNA is not completely denatured at 72°C. The annealing temperature is typically set to 5°C below the melting temperature (Tm) of the primers, which is usually between 50°C to 65°C, depending on the length and composition of the primers. The extension temperature is typically set to 72°C to ensure efficient amplification.