Answer:
When using the high power objective in a microscope, you should not adjust the coarse focus knob. The coarse focus knob is used to make large adjustments to the focus and is typically used when switching between objectives with different magnifications. When using the high power objective, small adjustments to the focus are made using the fine focus knob, which allows for precise control. Adjusting the coarse focus knob while using the high power objective can cause the specimen to be damaged or the objectives to become misaligned, resulting in poor image quality.
Step-by-step explanation: