Work is the change in energy
If we can find the initial state of energy and the final state of energy, we can see how much work we have to do
U= kq1q2/r
U = k(4.845x10^-9)(33.053x10^-9)/r
U = 1.44x10^-06/r
U(i) = 1.44x10^-06/5.384x10^-2 = 2.677x10^-5
U(f) = 1.44x10^-06/2.562x10^-2 = 5.626x10^-5
work = Uf - Ui = 2.949 x 10^-5 joules = 29.49 microJoules