The correct answer is false.
It is false that after succeeding Elizabeth I to the throne, James I changed her policies toward the Separatists.
Since Queen Elizabeth I, the separatist had represented a threat to the relative stability in the United Kingdom. Separatist wanted to have some degree of freedom to worship outside of the rigid structure of the Church of England. King James I of England, followed the policies of Queen Elizabeth I concerning the separatists. He showed no tolerance at all to them and to Catholics.