Question

Suppose you want to insert your pcr product into another organism's dna. which dna technique would you use?

Answer 1

Scientists use several techniques to manipulate DNA. DNA fragments are converted into fragments using electric current. First your DNA is amplified using PCR, then cut into your restriction enzymes.This gene usually will be inserted into other organism to produce a transgenic organism.DNA amplified by PCR may be sent for sequencing, visualized by gel electrophoresis or cloned.Blunt-ended fragments can be joined to each other by DNA ligase.

Answer 2

The correct answer would be recombinant DNA technology.

Recombinant DNA technology refers to the set of techniques used to combine the DNA fragments of different species or sources and then insert it into the host genome to produce the product of insert.

It can be achieved by producing the sticky ends in the gene of interest (PCR product) and host DNA (DNA of organism). The sticky ends are usually produced by digesting the two DNA(s) with the same restriction endonuclease.

Then they are allowed to recombine on the basis of the complementarity of the sticky ends.

Lastly, DNA ligase joins the strands of two DNA(s).