Question

Determine the correct sequence of events during the creation of recombinant DNA.I. Plasmid is cut open with enzymesII. Target gene is cut out with enzymesIII. Plasmid is inserted into the bacteriumIV. Target gene is bonded to the plasmid with LigaseA. IV, III, I, IIB. I, II, III, IVC. IV, I, II, IIID. II, I, IV, III

Answer 1

The correct answer is D. II, I, IV, III. After ligating target gene within the plasmid, it must be inserted into a bacterium.