no it does not
as long as you subtract the x's from the x's and y's from the y's
consider

and

alright, lets say x2-x1=z and x1-x2=-z
also y2-y1=t and y1-y2=-t
if we subsituted then we get

and

which simplifies to

and

the same thing
because the square of a negative becomes positive
so it doesn't matter