Any two of the following would do justice:
- DNA Helicase unwinds the double helix by disrupting H bonds that bind the nitrogenous bases
- Single-stranded binding proteins prevent the separated DNA strands from re-attaching
- RNA Primer made up of multiple nitrogenous bases which are attached to the template strand and initiate the building of a new complementary strand i.e replication
- Primase builds RNA primers
- DNA Polymerase I removes RNA primers and replaces them with appropriate DNA nucleotides
- DNA Polymerase III creates complementary strands of DNA, attach to an RNA primer, and add nucleotides to the 3' end
- DNA Ligase joins Okazaki fragments together by phosphodiester bonds
- DNA Gyrase relieves tension produced when DNA double helix is unwound by helicase