Question

Why did we make sure to include a start and stop dna sequences for the jellyfish?

Answer 1

Step-by-step explanation:

The DNA is composed of a sequence of nucleotides, each having a different base (adenine, thymine, guanine, cytosine). This DNA molecule is transcribed into RNA, which has a complementary sequence. Then the RNA will have a base sequence according to the information encoded in the DNA.

A codon consists of three bases together, which code for an amino acid. So each three-letter sequence of RNA corresponds to a specific amino acid, a start or stop codon.

AUG is the codon for methionine, and it is also the start codon, which indicates when the protein synthesis must begin. While UGA, UAA, and UAG are stop codons. Start and stop codons are important because then translation is not started or finished. During protein synthesis, stop codons cause the release of the protein from the ribosome.

So, that the protein synthesis begins and is carried out respecting the reading frame so that the appropriate protein is produced, and that it stops being synthesized when necessary, it is important to have a start and stop codon.

Answer 2

We did make sure to include a start and stop dna sequences for the jellyfish so that the Glo gene would be transcribed and expressed and so we can see that we have successfully transformed the cells into which we place the engineered plasmid.