37.3k views
2 votes
Which is the correct method for drawing liquid into a pipet?

User Danny Lo
by
5.5k points

1 Answer

2 votes
Allow the level of the fluid in the pipette to rise a couple centimeters above the line or mark on the side. While you are drawing up fluid make sure the the tip of the pipette always remains beneath the surface of the fluid. Do not allow the fluid to rise up into the bulb itself. Remove the bulb and quickly cap the open top of the pipette with your finger. By tilting your finger to one side, allow a little air into the pipette so the fluid drains out until the bottom of the meniscus (the curve-shaped depression in the top of the fluid) reaches the fill mark or line.
User Philcruz
by
6.0k points