When you expect something, but get something else, you have a reason big enough to ask new questions and start a new investigation.
Example:
If you create a tree by artificially breeding different species, and then plant it with certain expectations in width, size, bark width, leaf shape, etc. and things go differently, a new investigation might begin with a whole new load of questions.
Answer:
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Hope it helped,
Happy homewok/ study/ exam!