Question

You are in the lab trying to identify Protein X. The first gel you run for Protein X gives you one band at 30 kD and another band at 75 kD. You run another gel for Protein X using another technique. This time, you get one band at 210 kD. Circle below the possible techniques you used to obtain the above results.

Answer 1

SDS plus reducing agent in the first gel & SDS alone in the second gel

Step-by-step explanation:

• SDS-PAGE separates proteins primarily by mass because the ionic detergent SDS denatures and binds to proteins to make them uniformly negatively charged.
• Therefore when a current is applied, all SDS-bound proteins in a sample will move through the gel toward the positively charged electrode.