Question

Tumor-suppressor genes are normal human genes that prevent uncontrollable cell growth. Starting with a normal laboratory human cell line, describe how you could use transposon tagging to identify tumor-suppressor genes. (Note: When a TE hops into a tumor-suppressor gene, it may cause uncontrolled cell growth. This is detected as a large clump of cells among a normal monolayer of cells.)

Answer 1

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Step-by-step explanation:

First you need to develop a complete growth of human cells, in its natural state. Remember, you need to divide the group into the control one and the other that is going to go trough the treatment. Then, using the DNA data base, you need to encode the tumor suppressor and the genetic code of the tumor.

When you have both DNA, you need to take it into the normal cells (both groups), the DNA of the tumor to produce it.

When the cells present the unusual growing (cancer), it is time to introduce the other DNA sequence, so, it will observe how is the behavior of the tumor cells, if they stop reproducing the cancer cells or they continue the same.